Y. Ganesh Kumar

Research Supervisor, Department of Pharmaceutics, Bir Tikendrajit University, India


  • Research Article   
    Drug Development and Validation of a Sensitive Bio-analytical LCMS/MS Method for Quantification of Asciminib-a Chronic Myeloid Leukemia Drug in Human Plasma
    Author(s): Govindarao Yedlapalli* and Y. Ganesh Kumar

    This study targeted to develop a reliable and less time consuming Liquid Chromatography–Mass Spectrometry/Mass spectrometry (LC–MS/MS) method for quantification of chronic myeloid leukemia drug, asciminib in human plasma using sunitinib as internal standard. The analyte asciminib and sunitinib were extracted from spiked plasma by adopting liquid-liquid extraction using methyl tertiary butyl ether solvent and chromatographed on an Alltima HP C18 (100 mm × 4.6 mm, 3 μm) column with pH 4.3 ammonium formate (5 mM) buffer and methanol in 45 (v/v):55 (v/v) at pH 4.3 mL/min at 1.0 mL/min flow in isocratic mode. The analysis completed within a total chromatographic run time of 4 min that facilitates less time and solvent consumption. The column separated analytes were recorded using mass detector with positive ion electrospray ionization s.. Read More»
    DOI: 10.4303/JDAR/236242

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