A. Sai Datri

Department of Pharmaceutical Sciences, Andhra University College of Pharmaceutical Sciences, India


  • Research Article   
    RP-HPLC Method of Drug Development and Validation for the Determination of Ezetimibe using Design of Experiments Approach
    Author(s): A. Sai Datri*, K. S. Nataraj and A. Lakshmana Rao

    The present research aims to develop and validate a simple and accurate RP-HPLC method for the determination of Ezetimibe by using the Design of Experiments Approach. This approach was useful for multivariate optimization of the method. The critical method parameters (CMPs) were optimized using the Box-Behnken design. Minitab software was equipped for the study. Chromatographic separation was done on Phenomenex C18 column with specifications 150 mm × 4.6 mm × 5 μm at 30°C. The predicted and optimized data from the software consisted of mobile phase 0.02 N Ortho phosphoric acid (OPA) and Acetonitrile (53:47% v/v), pumped at a flow rate of 0.96 ml/min brought the desirability function of 1. The UV detector was adjusted at 232.6 nm. The developed method was shows linearity with a correlation coefficient of 0.999. The optimized chroma.. Read More»
    DOI: 10.4303/jdar/236198

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