In-Vivo Antinociceptive Activity and In-Silico Molecular Docking of Selected Phytoconstituents of Methanolic Extract of Hypericum Japonicum
Author(s): Shaik Aminabee*, G. Chandini Naga Mallika, M. Lakshmi Priya, K. Himaja Kasthuri, K. Harshitha, Shaherbanu, M. Mohansai, V. Adithya, Raveesha Peeriga and Lakshmana Rao Atmakuri
This research work was carried out to analyse and evaluate the antinociceptive activity of methanolic extract of Hypericum Japonicum (MEHJ) and in-silico molecular docking of selected phytoconstitutents with cyclooxygenase- 2 (COX-2) enzyme along with absorption (A), distribution (D), metabolism (M), excretion (E) and toxicity (T) studies. In-vivo antinociceptive activity was performed by hot plate method, tail immersion method and acetic acid induced writhing response method in rat. In-silico molecular docking was done by using Autodock Vina and Discovery Studio Visualizer. Absorption, distribution, metabolism, excretion and toxicity (ADMET) studies were examined by Swiss ADME software. The results proved that methanolic extract of Hypericum Japonicum has dose dependent antinoceptive activity at all doses. Among all the phytoconstitutents saroaspidin B has very best docking rate of -7.1 kcal/mol which was better virtually than standard celecoxib which has docking rate of -7.4 kcal/mol. This shows that there is good binding affinity between ligand and receptor than the standard i.e celecoxib. ADMET evaluation using swissADME and admeSAR software assures that saroaspidin B has followed all the 5 Lipinski’s guidelines suggesting that it is safety for consumption. Hence by this research, we conclude that Hypericum Japonicum can be a potent agent as antinociceptive activity and further studies are required to for the development of performance of saroaspidin B.