Evaluation of Antioxidant, Anti-inflammatory, Analgesic and Antipyretic activities of the Ethanolic Extract along with its Organic soluble fractions of Origanummajorana
Author(s): K. Ravishankar*, S. Nageswara Rao and V. Girija Sastry
Abstract
The present investigation involves the evaluation of antioxidant, analgesic, anti-inflammatory and antipyretic potential of the Ethanolic Extract along with its organic soluble fractions of Origanum majorana. Antioxidant potential of the extract/fractions was evaluated against various free radicals like Nitric oxide scavenging and Hydroxyl radicals using Ascorbic acid as standard. Ethanolic Extract along with its organic soluble fractions were evaluated for their analgesic activity by acetic acid-induced writhing, tail flick method and eddy`s hot plate method in albino rats. Further, the extract/fractions were studied for their Invitro anti-inflammatory(HRBC membrane stabilistaion and Protein denaturation), Invivo anti-inflammatory (carrageenan-induced paw oedema ) and antipyretic (Brewer’s yeast induced pyrexia) activities at a dose level of 200 and 400 mg/kg body weight for Ethanolic Extract /fractions. Ethyl acetate fractions (EtOAc) showed highest scavenging activity in all the methods with which isevident from their IC50 values 21.42 ug/mL in Nitric oxide scavengingmethod , 28.70 ug/mL in hydroxyl radical. Ethanolic Extract showed a dose dependent and significant analgesic ,anti-inflammatory (Invitro and invivo) and antipyretic effect. Dichloromethane fraction (CH2Cl2) and Ethyl acetate fractions exhibited similar activity in these models. The pharmacological effects exhibited by (CH2Cl2) fraction were lesser than the Ethanolic extract and ethylacetate fraction. In addition, total phenolic and flavonoid content were also determined. Altogether, these results suggest that the Ethanolic extract and its organic soluble fractions could be used as a potential antioxidant, analgesic, anti-inflammatory and antipyretic agent.