Anticancer Effect against Pancreatic Cell Line and Leukemia Cell Line Study of Scoparia Dulcis
Author(s): Shaik Harun Rasheed, S. Parthyusha*, K.H. Usha Devi, Ravi Kumar Vakkalagadda, K. Sumalatha, Ch. Suresh and P. Bharghava Bhushan Rao
Abstract
Cancer is a pathological state characterised by the uncontrolled proliferation and dissemination of abnormal cells originating from the body’s own tissues. Evaluating in-vitro cytotoxicity has become a widely used screening method for determining whether natural sources have anticancer potential. Utilising 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide, the MTT test was utilised to assess the antitumour efficaciousness of a hydroalcoholic extract obtained from Salvia dulcis L. Preliminary phytochemical analysis was also performed. To evaluate cell viability and inhibition, the MTT Assay was utilised. Cell viability decreased and growth inhibition increased in a concentrationdependent manner in the hydroalcoholic extract of Scoparia dulcis L. Additionally, it induced changes in cell morphology. S. dulcis IC50 values on Pancreatic and leukaemia cell lines were 104.56 g/ml and 73.51 g/ml, respectively, during a 24 hour incubation period, concentration range of 50 g to 150 g by MTT test. Doxorubicin was utilised as a standard control in the study. According to the findings, the hydroalcoholic extract of S. dulcis displays substantial anti-cancer effect on Pancreatic and leukaemia cell lines.
