Analysis of Drug Investigation of Anti Melanomal Effect of Ipomoea Pes-Tigridis L. Silver Nano Particles Produced By Green Synthesis Method through pharmaceutical scope
Author(s): Manukondu Bhaskar* and Kasoju Aruna
Abstract
The study aims to explore the green synthesis and therapeutic potential of silver nanoparticles (AgNPs) using the leaf extract of Ipomoea pes-tigridis L., a plant known for its diverse medicinal properties. Phytochemical screening was conducted to identify active compounds in the leaf extract responsible for nanoparticle synthesis and stabilization. Various analytical techniques, such as UV-visible spectroscopy, Fourier Transform Infrared (FT-IR) Spectroscopy, Scanning Electron Microscopy (SEM), particle analyzer, and X-Ray Diffraction (XRD), were employed to characterize the biosynthesized AgNPs. MTT assay was used to evaluate their cytotoxic potential. UV-visible spectrophotometry confirmed successful AgNP synthesis, with a distinct Surface Plasmon Resonance (SPR) band at 425 nm, indicative of small, spherical nanoparticles. FTIR analysis demonstrated the role of phytochemicals as capping and stabilizing agents. SEM and XRD analyses provided insights into particle size and crystal structure, revealing a face centered cubic lattice in the AgNPs. MTT assay was performed on 2 melanoma cell lines (A375 and B-16-F10) and Normal Human Dermal Fibroblasts (NHDF). The AgNPs displayed lower IC50 values than the leaf extract in cancer cell lines and demonstrated selective toxicity by showing higher IC50 values in normal cells. The study showcases the successful, green synthesis of AgNPs using Ipomoea pes-tigridis L. leaf extract. These AgNPs exhibited promising cytotoxic effects on melanoma cells and lower toxicity toward normal cells. The results establish the groundwork for further investigation into using such plant-derived nanoparticles for cancer treatments.