A Pilot Study of the Amelioration of Avocado Seed Oil in Obese Female Rats Induced by Carbon Tetrachloride and Alloxan Monohydrate

Author(s): Asmaa Fathi Hamouda* and Shifa Felemban


Obesity is a fat deposition pandemic with complications such as diabetes, inflammation, and hormonal and metabolic disturbances in the body. Obesity is associated with stress, energy imbalance, hormonal disturbances, drug, lifestyle, and genetic predispositions. There are various treatments for obesity including a lifestyle and an anti-obesity natural diet. We examined the bioeffects of dietary avocado (Persea americana) seeds extract (AVSE) against alloxan monohydrate-CCl4-induced obesity and a biochemical alteration. We, also, investigated the phytochemical contents of AVSE with gas chromatography mass spectrometry and inductively coupled plasma mass spectrometry. We study the beneficial effects of oral AVSE on the biological changes such as caspase-3, DNA fragmentation, prolactin, leptin, TSH (thyroid stimulating hormone), T3 (Triiodothyronine), total T4 (Thyroxine) and weight, diabetes, lipid and liver profiles caused by alloxan monohydrate and CCl4 in rats. The current outcomes reported the amelioration in the rats’ biochemical examination due to AVSE contents. The administration of AVSE after alloxan monohydrate and CCl4 injection significantly (p ≤ 0.05) modulated the studied analysis of caspase-3, prolactin, leptin, TSH, T3, total T4, total cholesterol, triglycerides, LDL-C, AST, ALT, glucose, and HDL levels by ↑ 42%, ↓35.4%, ↓ 40%, ↓ 13.6%, ↑ 18.2%, and ↑ 9207%, ↓ 17%, ↓ 35%, ↓ 10.9%, ↓ 34.7%, ↓ 34.3%, ↓ 51%, and ↑ 10.9 respectively, compared to injection with alloxan monohydrate and CCl4. AVSE had a healthy impact by decreasing the levels of caspase-3, DNA fragmentation, Prolactin, Leptin, TSH, T3, and total T4 and improving weight, diabetes, lipid, and liver profiles in rats. A further long term examination is required in progress to approve and validate the outcomes.

image 10.4303/jdar/236177

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